How To Incubate Leopard Gecko Eggs
There is a variety of methods for successfully incubating leopard gecko eggs. This is the method that I have been using. I have had a lot of success with it and I hope you will too.
Checklist
The first thing we should do is go over all everything we will need for the incubaation process. Here is the list of things that I use to incubate my Leopard Gecko eggs.
- 4.5” Deli Cup and Lid-Holes Punched: Fits two Leopard Gecko eggs nicely, I personally recommend you get them at TSK Supply. Click here for cup. (http://www.tsksupply.com/products/Pro-Kal-8-oz-CLEAR-PUNCHED.html) Click here for lid. ( http://www.tsksupply.com/pro-kal-lid-50-count/) The link should take you right to it. These are the cheapest I have been able to find them.
- Water Mister/Sprayer: Can be found at Walmart or a local nursery. MAKE SURE IT HAS NEVER BEEN USED BEFORE!! A used mister/sprayer might contain toxins that will kill or harm Leopard Gecko eggs.
- Organic Perlite: NO MIRACLE GROW PERLITE!!! Make sure it’s organic with nothing added to it. If it is not organic, it could harm or kill your eggs.
- Simple gram scale: Doesn’t have to be perfectly accurate. You will have to round up to the nearest gram anyway. You will also want your gram scale to have a tare or a zero out function.
- Simple Calculator: One that will add, subtract, multiply, and divide will do.
- Blue Painters’ Tape: For record keeping. This will come in handy later in the process.
- Thermal/Still Air Hova Bator Incubator 1602N: This is the best incubator for incubating Leopard Gecko eggs using this method. You can get one here. (http://geckosgalore.ecrater.com/p/13212414/thermal-hova-bator-incubator-1602n)
Method
First, take your 4.5” Deli Cup and put it on the gram scale and tare it so that the weight of the deli cup is zero. Once you have done that, fill the cup up about halfway with the organic perlite. Do not cover the holes. These will provide airflow throughout the incubation process.
Next, weigh the perlite.
Once you have weighed the cup with the perlite, take that weight and type it into your calculator. Multiply that weight by .666. So in this case, I would take 12 and multiply it by .666 which gives me 7.992 which rounds up to 8. That is the amount of water (in grams) that you will add to the perlite. In the example above you are going to add 8 to 12 to get 20. Here you would fill the perlite with water until the total weight on your scale is 20 grams.
(Even if your calculation of how much water to add comes out to be something like 7.336 or something like that you ALWAYS want to round UP to the next gram. The more humidity you have the better.)
Once you have done all of this, add your Leopard Gecko eggs. Put the lid on and put a piece of blue painters’ tape on the lid. Write the date the eggs were laid, the names of the parents, and the most important part, the weight of the perlite and water.
Date laid: (6/21/12)
Parents: (Striped Tremper Albino Male, Hybino Female)
Weight of water plus perlite: (20 grams)
Now put your eggs in the incubator. The sex of the geckos will be based on the incubation temperature:
(The following are rough guesses of how long the eggs will have to incubate before hatching)
- 81-83°, Mostly Females, roughly 65 days of incubation time.
- 84-86°, Mix of males and females, roughly 55 days of incubation time.
- 87-89°, Mostly Males, roughly 40 days of incubation time.
Do not incubate higher than 90 degrees! The eggs will hatch but the babies will be very aggressive females and MAY NOT breed! Don’t incubate under 80 because the eggs will get too cold and die.
VERY IMPORTANT!!!
Once your eggs are in the incubator, you should take the cup out of the incubator about every 4 days and weigh it again. If the weight doesn’t equal the weight listed on the cup (20 grams in this case) then you should brng it up to that weight again by adding water to it. Move the eggs to a different deli cup with perlite while you do this so you don’t accidentally get the eggs wet. When you are finished bringing the weight back up put the eggs back in and put the cup into the incubator. THIS IS A VERY IMPORTANT STEP, SO DON’T SKIP IT!
Final notes
I have used this method very successfully. Many of you might have read about other ways to incubate Leopard Gecko eggs, particularly recommending different water to perlite ratios. I have tried those methods for about a year and I never had an egg hatch. Of course, I am not saying that these methods are always unsuccessful, and that there could have been things wrong with what I was doing, however I have had my best success with the method I describe above.
If anyone has any questions, please leave a comment below.
Brian MagnussonVisit Website
Brian is the owner of Gecko's Galore. His passion for reptiles started back when he was a kid living in Missouri. He and his friends would spend our summer days going around the neighbor hood catching lizards and snakes. One of his friends had a Leopard Gecko and from the moment he saw it he knew he had to have one. His passion has grown from there.

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6 Responses
7.3.2012
Very well written article. Good job. Has the basic details needed to incubate leo eggs. I’ll refer people back to this article in the future.
7.4.2012
Excellent article; Well written with clear instructions and illustrations. Thank you for explaining it so well.
8.29.2012
Great article with ALL I need to know! Thanks again for everything. You’ve been so helpful. Quickly, (my daughter could tell me this, but she’s in bed) how often does a female lay eggs, and can you successfully put more than one female in a habitat with one male (ie 2 females with one male) or will they fight?
8.29.2012
@Kimberly, Females lay generally 2 eggs once a month throughout their breeding season which tends to be from February to the end of October. Some leos breeding seasons differ from others. Usually if it is a first time egg laying female she will only lay 1 egg and that egg will be infertile.
You can put any number of females in with a male. NEVER put more then one male in the same cage. They will fight, which could result in death. I put 1 male with 2-3 females depending on how big my cage is. I put my leos in a rack system. I put my single adults in a 12 qt sterilite tub and 1 male with 2 females in a 28 qt sterilite tub. I put my single babies and juveniles in 6 qt sterilite tubs.
You asked a question earlier when you were emailing me. You asked me what a genetic mutt is. A genetic mutt is a Leopard Gecko that you breed where you don’t know either of the parents morphs you bred together to produce those babies. A lot of Leopard Gecko breeders look down on breeding your geckos if you don’t know what morph they are. Here is another example of a genetic mutt. There are 3 kinds of albinos in Leopard Geckos. Tremper Albino, Bell Albino, and Rainbow Albino (also known as the Las Vegas Albino.) The albinos in Leopard Geckos are not compatible which means they shouldn’t be bred to each other. An example of that would be breeding a Tremper Albino to a Bell Albino. That just messes up the gene pool, like in dogs.
I hope that I put that so that you could understand it. When I start talking gecko genetic talk or any gecko talk, I get carried away.
You can email me or comment on this article if you have anymore questions. Since that was just the summary of Leopard Gecko Genetics.
Thanks
Brian Magnusson
Gecko’s Galore
9.14.2012
I have noticed here recently, that my Leo gecko eggs have
Been growing white fuzzy mold. What does this usually mean
And how can I prevent it? I am using Hatchrite substrate. If anyone
Has any suggestions that would be of great help!
Thanks so much!
9.15.2012
Hi Jennifer!
This usually means that the humidity levels are to high. I would recommend changing your substrate to perlite and using this method entirely. If you do it right, the humidity levels should be perfect.
Let me know if you have anymore questions!